منابع مشابه
High-affinity binding of [3H]acetylcholine to muscarinic cholinergic receptors.
High-affinity binding of [3H]acetylcholine to muscarinic cholinergic sites in rat CNS and peripheral tissues was measured in the presence of cytisin, which occupies nicotinic cholinergic receptors. The muscarinic sites were characterized with regard to binding kinetics, pharmacology, anatomical distribution, and regulation by guanyl nucleotides. These binding sites have characteristics of high-...
متن کاملOccurrence of muscarinic acetylcholine receptors in wild type and cholinergic mutants of Caenorhabditis elegans.
Crude homogenates of the nematode worm Caenorhabditis elegans were shown to bind the cholinergic antagonists [3H]N-methylscopolamine and quinuclidinyl benzilate ([3H]QNB) with high affinity. The dissociation constant for [3H]N-methylscopolamine binding determined from equilibrium saturation experiments (KD = 3.7 x 10-l’ M) was in good agreement with that determined from forward and reverse rate...
متن کاملRegulation of human alpha4beta2 neuronal nicotinic acetylcholine receptors by cholinergic channel ligands and second messenger pathways.
The alpha4beta2 nicotinic acetylcholine receptors (nAChRs), a major subtype in the brain, have been shown to be modulated by chronic treatment with nicotine. In this study, the regulation of recombinant human alpha4beta2 nAChR subtype by (-)-nicotine and other cholinergic channel modulators was studied using human embryonic kidney 293 cells stably expressing this subunit combination. The treatm...
متن کاملCholinergic Modulation of Locomotion and Striatal Dopamine Release is Mediated by α6α4* Nicotinic Acetylcholine Receptors
Dopamine (DA) release in striatum is governed by firing rates of midbrain DA neurons, striatal cholinergic tone, and nicotinic ACh receptors (nAChRs) on DA presynaptic terminals. DA neurons selectively express α6* nAChRs, which show high ACh and nicotine sensitivity. To help identify nAChR subtypes that control DA transmission, we studied transgenic mice expressing hypersensitive α6L9′S* recept...
متن کاملRegulation of Human a4b2 Neuronal Nicotinic Acetylcholine Receptors by Cholinergic Channel Ligands and Second Messenger Pathways
The a4b2 nicotinic acetylcholine receptors (nAChRs), a major subtype in the brain, have been shown to be modulated by chronic treatment with nicotine. In this study, the regulation of recombinant human a4b2 nAChR subtype by (2)-nicotine and other cholinergic channel modulators was studied using human embryonic kidney 293 cells stably expressing this subunit combination. The treatment of transfe...
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ژورنال
عنوان ژورنال: Brain and Neuroscience Advances
سال: 2019
ISSN: 2398-2128,2398-2128
DOI: 10.1177/2398212818820506